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090			_aB-7817
245	1	0	_aIdenti¢cation of a possible MAP kinase cascade in Arabidopsis thaliana based on pairwise yeast two-hybrid analysis and functional complementation tests of yeast mutants
490	0		_vFEBS Letters, 437, p.56-60, 1998
520	3		_aA possible MAP kinase (MAPK)cascade of Arabidopsis thaliana was identified on the basis of both yeast 2-hybrid analysis and complementation analysis of yeast mutants. Specific protein-protein interactions between ATMPK4 (a MAPK)and MEK1 (a MAPKK)and interactions between MEK1 and ATMEKK1 (a MAPKKK)were detected by using the 2-hybrid system. A growth defect of the yeast mpk1v mutant was reversed by coexpression of ATMPK4 and MEK1. Coexpression of the N-terminal deletion form of ATMEKK1 increased the ability of MEK1 to suppress a growth defect of the yeast pbs2v mutant. These results suggest that ATMPK4, MEK1, and ATMEKK1 may interact with each other and constitute a specific MAPK cascade in Arabidopsis. This is the first demonstration of a possible MAPK cascade in plants.
650	1	4	_aMITOGEN-ACTIVATED PROTEIN KINASE CASCADE
650	1	4	_aYEAST TWO-HYBRID
650	1	4	_aTRANS-COMPLEMENTATION
650	1	4	_aARABIDOPSIS THALIANA
700	1	2	_aMizoguchi, T.
700	1	2	_aIchimura, K.
700	1	2	_aIrie, K.
700	1	2	_aMorris, P.
700	1	2	_aGiraudat, J.
700	1	2	_aMatsumoto, K.
700	1	2	_aShinozaki, K.
856	4	0	_uhttps://drive.google.com/file/d/1NqgOm4YG9w44icEbG7g-rAC-p25j4iCl/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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