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245 1 0 _aElectrochemical immunoassay of estrone at an antibody-modified conducting polymer electrode towards immunobiosensors
490 0 _vJournal of Electroanalytical Chemistry, 592(1), p.88-94, 2006
520 3 _aA simple electrochemical immunoassay based on an antibody-modified conducting polymer electrode by direct electrochemical polymerization of pyrrole on a platinum electrode in an aqueous solution and simultaneous doping of the polymer with anti-estrone antibody as the dopant was demonstrated. The specific binding between estrone and anti-estrone antibody immobilized on the electrode surface was evaluated by monitoring the changes in the electrode response of potassium ferrocyanide as the redox marker and a decreased electrode response was observed upon the binding of the estrone to the polypyrrole-antibody (PPy-Ab)modified electrode surface. This change was attributed to the steric hindrance generated from the formation of immunological complex between the electrode surface and the redox marker. A direct and facile method of electrochemical detection of estrone has been established in this way.
650 1 4 _aESTRONE
650 1 4 _aPOLYPYRROLE
650 1 4 _aFERROCYANIDE
650 1 4 _aIMMUNOASSAY
650 1 4 _aELECTROCHEMICAL DETECTION
700 1 2 _aGao, H.
700 1 2 _aLu, J.
700 1 2 _aCui, Y.
700 1 2 _aZhang, X.X.
856 4 0 _uhttps://drive.google.com/file/d/1t-DyQFEOtVIYk8FW9VoBlECtBqzGaNE4/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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