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245	1	0	_aProteome analysis of hairy root from Panax ginseng C. A. Meyer using peptide fingerprinting, internal sequencing and expressed sequence tag data
490	0		_vProteomics, 3(12), p.2379-2392, 2003
520	3		_aAs an initial step to the comprehensive proteomic analysis of Panax ginseng C. A. Meyer, protein mixtures extracted from the cultured hairy root of Panax ginseng were separated by two-dimensional polyacrylamide gel electrophoresis (2-DE). The protein spots were analyzed and identified by peptide finger printing and internal amino acid sequencing by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS)and electrospray ionization quadrupole-time of flight mass spectrometry (ESI Q-TOF MS), respectively. More than 300 protein spots were detected on silver stained two-dimensional (2-D)gels using pH 3-10, 4-7, and 4.5- 5.5 gradients. Major protein spots (159)were analyzed by peptide fingerprinting or de novo sequencing and the functions of 91 of these proteins were identified. Protein identification was achieved using the expressed sequence tag (EST)database from Panax ginseng and the protein database of plants like Arabidopsis thaliana and Oryza sativa. However, peptide mass fingerprinting by MALDI-TOF MS alone was insufficient for protein identification because of the lack of a genome database for Panax ginseng. Only 17 of the 159 protein spots were verified by peptide mass fingerprinting using MALDI-TOF MS whereas 87 out of 102 protein spots, which included 13 of the 17 proteins identified by MALDI-TOF MS, were identified by internal amino acid sequencing using tandem mass spectrometry analysis by ESI Q-TOF MS. W ten the internal amino acid sequences were used as identification markers, the identification rate exceeded 85.3
650	1	4	_aEXPRESSED SEQUENCE TAG DATA
650	1	4	_aHAIRY ROOT
650	1	4	_aINTERNAL SEQUENCING
650	1	4	_aPANAX GINSENG
650	1	4	_aC. A. MEYER
650	1	4	_aPEPTIDE FINGERPRINTING
650	1	4	_aPROTEOME ANALYSIS
700	1	2	_aKim, S.I.
700	1	2	_aJin Young Kim
700	1	2	_aEun A. Kim
700	1	2	_aKyung-Hoon Kwon
700	1	2	_aKyung-Wook Kim
700	1	2	_aKun Cho
700	1	2	_aJeong Hwa Lee
700	1	2	_aJeong Hwa Lee
700	1	2	_aDeok-Chun Yang
700	1	2	_aJong Shin Yoo
700	1	2	_aYoung Mok Park
856	4	0	_uhttps://drive.google.com/file/d/18GrJZJQcEgGB-3t0M-XrdbnITUl8NVgZ/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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