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245	1	0	_aThe role of phloem sieve elements and laticifers in the biosynthesis and accumulation of alkaloids in opium poppy
490	0		_vPlant Journal, 47(4), p.547-563, 2006
520	3		_aThe benzylisoquinoline alkaloids of opium poppy, including the narcotic analgesics morphine and codeine, accumulate in the multinucleate cytoplasm of specialized laticifers that accompany vascular tissues throughout the plant. In mature opium poppy plants, immunofluorescence labeling using specific antibodies showed that four alkaloid biosynthetic enzymes, (S)-norcoclaurine 6-O-methyltransferase (6OMT), (S)-coclaurine N-methyltransferase (CNMT), (S)-3¢-hydroxy-N-methylcoclaurine-4¢-O-methyltransferase (4¢OMT)and alutaridinol-7-O-acetyltransferase (SAT)were restricted to sieve elements of the phloem adjacent or proximal to laticifers. The identity of sieve elements was confirmed by (i)the specific immunogold labeling of the characteristic cytoplasm of this cell type, (ii)the co-localization of a sieve element-specific Hþ-ATPase with all biosynthetic enzymes and (iii)the strict association of sieve plates with immunofluorescent cells. The localization of laticifers was demonstrated antibodies specific to major latex protein (MLP), which is characteristic of this cell type. In situ hybridization using antisense RNA probes for 6OMT, CNMT, 4¢OMT and SAT showed that the corresponding gene transcripts were found in the companion cell paired with each sieve element. Seven benzylisoquinoline alkaloid biosynthetic enzymes, (S)-N-methylcoclaurine 3¢-hydroxylase (CYP80B1), berberine bridge enzyme, codeinone reductase, 6OMT, CNMT, 4¢OMT and SAT were localized by immunofluorescence labeling to the sieve elements in the root and hypocotyl of opium poppy seedlings. The abundance of these enzymes increased rapidly between 1 and 3 days after seed germination. The localization of seven biosynthetic enzymes to the sieve elements provides strong support for the unique, cell type-specific biosynthesis of benzylisoquinoline alkaloids in the opium poppy.
650	1	4	_aBENZYLISOQUINOLINE ALKALOIDS
650	1	4	_aIMMUNOFLUORESCENCE LABELING
650	1	4	_aIMMUNOGOLD LABELING
650	1	4	_aIN SITU HYBRIDIZATION
650	1	4	_aPAPAVER SOMNIFERUM
650	1	4	_aSECONDARY METABOLISM
700	1	2	_aSamanani, N.
700	1	2	_aAlcantara, J.
700	1	2	_aBourgault, R.
700	1	2	_aZulak, K.G.
700	1	2	_aFacchini, P.J.
856	4	0	_uhttps://drive.google.com/file/d/1nkwiaTs-gcUrVa_wgVOes28_aBXL9h8i/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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