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090			_aB-7982
245	1	0	_aRapid identification of non-essential genes for in vitro replication of Marek's disease virus by random transposon mutagenesis
490	0		_vJournal of Virological Methods, 135(2), p.288-291, 2006
520	3		_aMarek's disease virus (MDV)is a highly oncogenic alphaherpesvirus that induces rapid-onset T-cell lymphomas in poultry. The MDV genome encodes more than 100 genes. However, the role of many of these genes in virus replication is not known. The construction of an infectious bacterial artificial chromosome (BAC)clone of the highly oncogenic RB-1B strain ofMDV has been described previously. Virus reconstituted from the BAC clone induced rapid-onset lymphomas in chickens very similar to the wildtype viruses. In this paper, the construction of a high-density random transposon-insertion mutant library of the RB-1B BAC clone using a high throughput in vitro transposon mutagenesis technique is described. Furthermore a PCR screening method, using primers specific for the transposon sequence and the MDV gene(s)of interest, was developed for the rapid identification of specific insertion mutants. The application of the screening method to identify some of the non-essential genes for MDV replication in vitro is described.
650	1	4	_aMDV
650	1	4	_aBAC
650	1	4	_aTRANSPOSON MUTAGENESIS
700	1	2	_aChattoo, J.P.
700	1	2	_aStevens, M.P.
700	1	2	_aNair, V.
856	4	0	_uhttps://drive.google.com/file/d/17FW5t-TAOVFULME82eLGXfnFPiRJ9sQ2/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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