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245 1 0 _aImmediate-early and delayed cytokinin response genes of Arabidopsis thaliana identified by genome-wide expression profiling reveal novel cytokinin-sensitive processes and suggest cytokinin action through transcriptional cascades
490 0 _vThe Plant Journal, 44(2), p.314-333, 2005
520 3 _awork has revealed that the cytokinin signal is transduced by two-component systems to the nucleus where target genes are activated. Most of the rapid transcriptional responses are unknown. We measured immediateearly and delayed cytokinin responses through genome-wide expression profiling with the Affymetrix ATH1 full genome array (Affymetrix Inc., Santa Clara, CA, USA). Fifteen minutes after cytokinin treatment of 5-dayold Arabidopsis seedlings, 71 genes were upregulated and 11 genes were downregulated. Immediate-early cytokinin response genes include a high portion of transcriptional regulators, among them six transcription factors that had previously not been linked to cytokinin. Five plastid transcripts were rapidly regulated as well, indicating a rapid transfer of the signal to plastids or direct perception of the cytokinin signal by plastids. After 2 h of cytokinin treatment genes coding for transcriptional regulators, signaling proteins, developmental and hormonal regulators, primary and secondary metabolism, energy generation and stress reactions were overrepresented. A significant number of the responding genes are known to regulate light (PHYA, PSK1, CIP8, PAT1, APRR), auxin (Aux/IAA), ethylene (ETR2, EIN3, ERFs/EREBPs), gibberellin (GAI, RGA1, GA20 oxidase), nitrate (NTR2, NIA)and sugar (STP1, SUS1)dependent processes, indicating intense crosstalk with environmental cues, other hormones and metabolites. Analysis of cytokinin-deficient 35S:AtCKX1 transgenic seedlings has revealed additional, long-lasting cytokinin-sensitive changes of transcript abundance. Comparative overlay-analysis with the software tool MAPMAN identified previously unknown cytokinin-sensitive metabolic genes, for example in the metabolism of trehalose-6-phosphate. Taken together, we present a genome-wide view of changes in ytokinin-responsive transcript abundance of genes that might be functionally relevant for the many biological processes that are governed by cytokinins.
650 1 4 _aCYTOKININ
650 1 4 _aGENE EXPRESSION
650 1 4 _aIMMEDIATE-EARLY GENES
650 1 4 _aRESPONSE GENES
650 1 4 _aTRANSCRIPTOME ANALYSIS.
700 1 2 _aBrenner, W.G.
700 1 2 _aRomanov, G.A.
700 1 2 _aKollmer, I.
700 1 2 _aBurkle, L.
700 1 2 _aSchmulling, T.
856 4 0 _uhttps://drive.google.com/file/d/1i45E3ugsWyQWqUBtzBd6ZSlhFM8GtYnl/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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