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090			_aB-8076
245	1	0	_aTrehalose-6-phosphate synthase as an intrinsic selection marker for plant transformation
490	0		_vJournal of Biotechnology, 121(3), p.309-317, 2006
520	3		_aInsertion of foreign DNA into plant genomes occurs randomly and with low frequency. Hence, a selectable marker is generally required to identify transgenic plants. Until now, all selection systems have been based on the use of non-plant genes, derived from microorganisms and usually conferring antibiotic or herbicide resistance. The use of microorganism-derived genes however has raised biosafety concerns.We have developed a novel selection system based on enhancing the expression of a plant-intrinsic gene and the use of a harmless selection agent. Selection takes advantage of the reduced glucose sensitivity of seedlings with enhanced expression of AtTPS1, a gene encoding trehalose-6-P synthase. As a result, transformants can be identified as developing green seedlings amongst the background of small, pale non-transformed plantlets on high glucose medium. In addition, vegetative regeneration of tobacco leaf explants is very sensitive to high external glucose. Overexpression of AtTPS1 in tobacco allows selecting glucose insensitive transgenic shoots.
650	1	4	_aPLANT TRANSFORMATION
650	1	4	_aINTRINSIC SELECTION MARKER
650	1	4	_aATTPS1
650	1	4	_aTREHALOSE-6-PHOSPHATE SYNTHASE
650	1	4	_aGLUCOSE RESISTANCE
650	1	4	_aARABIDOPSIS THALIANA
650	1	4	_aNICOTIANA TABACUM
700	1	2	_aLeymana, B.
700	1	2	_aAvonce, N.
700	1	2	_aRamona, M.
700	1	2	_aVan Dijck, P.
700	1	2	_aIturriaga, G.
700	1	2	_aThevelein, J.M.
856	4	0	_uhttps://drive.google.com/file/d/1BbjSohvbIc-4mn63isCf7YsjGsr30uv-/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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