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| 003 | MX-MdCICY | ||
| 005 | 20250625124714.0 | ||
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| 090 | _aB-8116 | ||
| 245 | 1 | 0 | _aBiochemical Characterization of the First Essential Two-Component Signal Transduction System from Staphylococcus aureus and Streptococcus pneumoniae |
| 490 | 0 | _vJournal of Molecular MicroBiology and Biotechnology, 5(4), p.252-260, 2003 | |
| 520 | 3 | _aThe yycFG two-component signal transduction system (TCSTS)has been shown to be essential to the viability of several gram-positive bacteria. However, the function of the gene pair remains unknown. Interestingly, while both components are essential to Staphylococcus aureus and Bacillus subtilis, only the response regulator (yycF)is essential to Streptococcus pneumoniae. To study this essential TCSTS further, the S. pneumoniae and S. aureus truncated YycG histidine kinase and full-length YycF response regulator proteins were characterized at a biochemical level. The recombinant proteins from both organisms were expressed in Escherichia coli and purified. The YycG autophosphorylation activities were activated by ammonium. The apparent Km (ATP)of S. aureus YycG autophosphorylation was 130 ÌM and S. pneumoniae was 3.0 ÌM. Each had similar kcat values of 0.036 and 0.024 min-1, respectively. Cognate phosphotransfer was also investigated indicating different levels of the phosphorylated YycG intermediates during the reaction. The S. pneumoniae YycG phosphorylated intermediate was not detectable in the presence of its cognate YycF, while phosphorylated S. aureus YycG and YycF were detected concurrently. In addition, noncognate hosphotransfer was demonstrated between the two species. These studies thoroughly compare the essential YycFG TCSTS from the two species at the biochemical level and also establish methods for assaying the activities of these antibacterial targets. | |
| 650 | 1 | 4 | _aYYCF |
| 650 | 1 | 4 | _aYYCG |
| 650 | 1 | 4 | _aRESPONSE REGULATOR |
| 650 | 1 | 4 | _aHISTIDINE KINASE |
| 700 | 1 | 2 | _aClausen, V.A. |
| 700 | 1 | 2 | _aBae, W. |
| 700 | 1 | 2 | _aThroup, J. |
| 700 | 1 | 2 | _aBurnham, M.K.R. |
| 700 | 1 | 2 | _aRosenberg, M. |
| 700 | 1 | 2 | _aWallis, N.G. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1A0vDO__ht5JrZLCwAmwza9j7mZ33_NJu/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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