000			01821nam a2200253Ia 4500
003			MX-MdCICY
005			20250625124714.0
040			_cCICY
090			_aB-8133
245	1	0	_aVisualizing Plant Development and Gene Expression in Three Dimensions Using Optical Projection Tomography
490	0		_vThe Plant Cell, 18(9), p.2145-2156, 2006
520	3		_aA deeper understanding of the mechanisms that underlie plant growth and development requires quantitative data on threedimensional (3D)morphology and gene activity at a variety of stages and scales. To address this, we have explored the use of optical projection tomography (OPT)as a method for capturing 3D data from plant specimens. We show that OPT can be conveniently applied to a wide variety of plant material at a range of scales, including seedlings, leaves, flowers, roots, seeds, embryos, and meristems. At the highest resolution, large individual cells can be seen in the context of the surrounding plant structure. For naturally semitransparent structures, such as roots, live 3D imaging using OPT is also possible. 3D domains of gene expression can be visualized using either marker genes, such as b-glucuronidase, or more directly by whole-mount in situ hybridization. We also describe tools and software that allow the 3D data to be readily quantified and visualized interactively in different ways.
700	1	2	_aLee, K.
700	1	2	_aAvondo, J.
700	1	2	_aMorrison, H.
700	1	2	_aBlot, L.
700	1	2	_aStark, M.
700	1	2	_aSharpe, J.
700	1	2	_aBangham, A.
700	1	2	_aBangham, A.
856	4	0	_uhttps://drive.google.com/file/d/1xYnKdARjnYXgz2n1Qsbqe-txniEU4lqq/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c42441 _d42441