000			02036nam a2200241Ia 4500
003			MX-MdCICY
005			20250625124715.0
040			_cCICY
090			_aB-8153
245	1	0	_aCytotoxic and anti-tumour properties of certain taxa of Umbelliferae with special reference to Centella asiatica (L.)Urban
490	0		_vJournal of EthnoPharmacology, 48(1), p.53-57, 1995
520	3		_aCentella asiatica (L.)Urban (Umbelliferae)is commonly used in the Ayurvedic system of medicine to treat various diseases. The present study examines the anti-tumour effect of the crude extract (CE)of Centella asiatica as well as its partially purified fractions (AF)from chromatographic procedures by both in vitro short and long term chemosensitivity and in vivo tumour model test systems. AF dose dependently inhibited the proliferation of the transformed cell lines significantly more than did the CE and other solvent fractions. Fifty percent effective doses on hour 3 exposure to AF were 17 and 22 #g/ml for Ehrlich ascites tumour cells (EAC)and Dalton's lymphoma ascites tumour cells (DLA), respectively. However, practically no toxic effects were detected in normal human lymphocytes. AF also significantly suppressed the multiplication of mouse lung fibroblast (L-929)cells at a concentration of 8 t~g/ml in long term culture. Oral administration of the extracts (CE and AF)retarded the development of solid and ascites tumours and increased the life span of these tumour bearing mice. Tritiated thymidine, uridine and leucine incorporation assay suggest that the fraction acts directly on DNA synthesis.
650	1	4	_aCENTELLA ASIATICA
650	1	4	_aUMBELLIFERAE
650	1	4	_aCYTOTOXICITY
650	1	4	_aANTI-TUMOUR
700	1	2	_aBabu, T.D.
700	1	2	_aKuttan, G.
700	1	2	_aPadikkala, J.
856	4	0	_uhttps://drive.google.com/file/d/1-6QCocL7QeF9tXaygPmxjKYgKN5VcyrX/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c42460 _d42460