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245 1 0 _aDifferential expression of 10 sweetpotato peroxidase genes in response to bacterial pathogen, Pectobacterium chrysanthemi
490 0 _vPlant Physiology and BioChemistry, 42(5), p.451-455, 2004
520 3 _aTo understand the function of each peroxidase (POD, EC 1.11.1.7)in terms of biotic stress, changes in POD specific activity and expression of 10 POD genes were investigated in four cultivars of sweetpotato (Ipomoea batatas)after infection with Pectobacterium chrysanthemi. POD specific activity (units mg-1 protein)increased from 16 h after inoculation (HAI)in three varieties. POD activities of two cultivars, Shinwhangmi and White Star, reached a maximum level at 24 HAI by about three times compared to mock treatment (MT), and then decreased, whereas those of Zami andYulmi continuously increased until 36 HAI. Native gel analysis revealed that one POD isoenzyme with a high electrophoretic mobility significantly increased in response to pathogen infection in all cultivars. Additionally, 10 POD genes displayed differential expression patterns upon bacterial infection by northern analysis. Several POD genes such as swpa2, swpa3, swpa4, swpa5, swpb1 were induced upon bacterial infection, but other genes were not. Particularly, swpa4 gene was markedly expressed in response to bacterial infection in four different cultivars, suggesting that this gene has a stress-inducible promoter. These results indicate that some specific POD isoenzymes are involved in defense in relation to pathogenesis of P. chrysanthemi in sweetpotato plants.
650 1 4 _aIPOMOEA BATATAS
650 1 4 _aBIOTIC STRESS
650 1 4 _aCONVOLVULACEAE
650 1 4 _aNATIVE GEL ANALYSIS
650 1 4 _aNORTHERN ANALYSIS
650 1 4 _aPEROXIDASE
650 1 4 _aPECTOBACTERIUM CHRYSANTHEMI
700 1 2 _aJang, I.C.
700 1 2 _aPark, S.Y.
700 1 2 _aKim, K.Y.
700 1 2 _aKwon, S.Y.
700 1 2 _aKim, J.G.
700 1 2 _aKwak, S.S.
856 4 0 _uhttps://drive.google.com/file/d/1tMak6NQlE0SUTx1wtLuYkRUdobVnpmqg/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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