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245 1 0 _aOxidative stress in recalcitrant tissue cultures of grapevine
490 0 _vFree Radical Biology & Medicine, 16(3), p.355-362, 1994
520 3 _aThiobarbituric acid reactive substances (TBARS), and fluorescent compounds with spectral characteristics typical of products associated with oxidative stress in senescent and aging plant and animal cells, were detected in tissue cultures of the recalcitrant grapevine Vitis vinifera L. cultivar, Sultanina. These compounds increased during the early stages of dedifferentiation (callogenesis)of nodal stem explants. Catalase activity was not detected in the original explant, but was induced during callogenic dedifferentiation. Conversely, superoxide dismutase activity was detectable in the original explant, but diminished during the first week of callus induction. Transfer to callus induction medium promoted a large increase in the sulfhydryl content of nodal tissues. TBARS and fluorescent products accumulated in Sultanina callus during long-term culture (over 6 months). The possibility that oxidative stress may contribute to culture recalcitrance in this vine is discussed.
650 1 4 _aFREE RADICALS
650 1 4 _aANTIOXIDANTS
650 1 4 _aVITIS VINIFERA L.
650 1 4 _aLIPID PEROXIDATION
650 1 4 _aMALONDIALDEHYDE
650 1 4 _aCATALASE
650 1 4 _aSUPEROXIDE DISMUTASE
650 1 4 _aTISSUE CULTURE
700 1 2 _aBenson, E.E.
700 1 2 _aRoubelakis-Angelakis, K.A.
856 4 0 _uhttps://drive.google.com/file/d/1d1L4av4Nfl_o5iEBItO-SDf0Y816hL0B/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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