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245 1 0 _aMass propagation of conifer trees in liquid cultures-progress towards commercialization
490 0 _vPlant Cell, Tissue and Organ Culture, 81, p.339-346, 2005
520 3 _aAt Weyerhaeuser Company, somatic embryo production in liquid medium and manufactured seed delivery has been developed to reduce labor costs and increase efficiency of mass clonal propagation. We have scaled-up embryonal suspensor masses (ESM)of Douglas-fir in 1-l liquid medium flasks. Over 10,000 somatic embryos have been produced from a single flask. We have cryostored ESM of over 700 genotypes in liquid nitrogen. Somatic embryos of Douglas-fir have also been produced from liquid medium in a bioreactor. Different types of bioreactors are required for embryo multiplication and for cotyledonary embryo development. Over 250,000 Douglas-fir somatic seedlings from a large number of enotypes have been produced for clonal field tests.
650 1 4 _aABA
650 1 4 _aABSCISIC ACID
650 1 4 _aESM-EMBRYONAL SUSPENSOR MASS
650 1 4 _aGA-GIBBERELLIC ACID
650 1 4 _aPEG-POLYETHYLENE GLYCOL
700 1 2 _aGupta, P.K.
700 1 2 _aTimmis, R.
856 4 0 _uhttps://drive.google.com/file/d/1GoXFxyQQRFEW3tRdn4psnC9jgyLSptTe/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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