000			02704nam a2200361Ia 4500
003			MX-MdCICY
005			20250625124719.0
040			_cCICY
090			_aB-8410
245	1	0	_aAntimicrobial peptides from chilli pepper seeds causes yeast plasma membrane permeabilization and inhibits the acidification of the medium by yeast cells
490	0		_vBiochimica et Biophysica Acta, 1760(9), p.1323-1332, 2006
520	3		_aDuring the last few years, a growing number of cysteine-rich antimicrobial peptides has been isolated from plants and particularly from seeds. It has become increasingly clear that these peptides play an important role in the protection of plants against microbial infection. In this work, proteins from chilli pepper (Capsicum annuum L.)seeds were extracted in phosphate buffer, pH 5.4 and peptides purification were performed by employing ion-exchange chromatographies on DEAE, CM-Sepharose, Sephacryl S-100 and reverse phase in HPLC. Three peptide enriched fractions, namely F1, F2 and F3, were obtained after the CM-Sepharose chromatography. The F1 fraction, mainly composed of three peptides ranging from 6 to 10 kDa, was submitted to N-terminal amino acid sequencing. The closer to 10 kDa peptide showed high sequence homology to lipid transfer proteins (LTPs)previously isolated from others seeds. F1 fraction exhibited strong fungicidal activity against Candida albicans, Saccharomyces cerevisiae and schizosaccharomyces pombe and also promoted several morphological changes to C. albicans, including the formation of pseudohyphae, as revealed by scanning electron micrography. F1 fraction also reduced the glucose stimulated acidification of the medium mediated by H+-ATPase of S. cerevisiae cells in a dose-dependent manner and caused the permeabilization of yeast plasma membrane to the dye SYTOX Green, as verified by confocal laser microscopy.
650	1	4	_aLIPID TRANSFER PROTEIN
650	1	4	_aMEMBRANE PERMEABILIZATION
650	1	4	_aCAPSICUM ANNUUM
650	1	4	_aPLANT ANTIMICROBIAL PEPTIDE
650	1	4	_aSACCHAROMYCES CEREVISIAE
650	1	4	_aCANDIDA ALBICANS
700	1	2	_aDiz, M.S.S
700	1	2	_aCarvalho, A.O.
700	1	2	_aRodrigues, R.
700	1	2	_aNeves-Ferreira, A.G.C.
700	1	2	_aDa Cunha, M.
700	1	2	_aWalter Alves, E.
700	1	2	_aOkorokova-Façanha, A.L.
700	1	2	_aOkorokova-Façanha, A.L.
700	1	2	_aPerales, J.
700	1	2	_aMachado, O.L.T.
700	1	2	_aGomes, V.M.
856	4	0	_uhttps://drive.google.com/file/d/1ckQFTc3lHMf-J9bfzzjo5DprBGP89Vh_/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c42714 _d42714