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090			_aB-8435
245	1	0	_aEarly.flowering Scots pines through tissue culture for accelerating tree breeding
490	0		_vTheoretical and Applied Genetics, 93(5-6), p.840-848, 1996
520	3		_aScots pine plantlets were produced via tissue culture using cotyledons excised from germinated embryos as ~xplants. The optimum tissue culture conditions were: ~4GD basal medium gelled with agar-Gelrite during shoot formation and with agar during rooting, inclusion of 5.0 btM benzylaminopurine (BAP)and 0.05 pM naphthaleneacetic acid (NAA)for 2 weeks for shoot induction, and repeated 2.7 pM NAA pulses of 1 week for rooting. Micropropagation success was genotype-dependent. Average multiplication rates varied among experiments from 3 to 15 shoots per embryo. The maximum shoot production from a single embryo was 35. Rooting was the most difficult phase in the propagation process. Most of the plantlets had a plagiotrophic and highly branched growth habit when growing in the greenhouse. Some individuals produced megasporangiate strobili at the age of 3 years and microsporangiate strobili with viable pollen at the age of 4 years. Early-flowering clones and the ability to onserve seedlings from which cotyledons have been cultured give new possibilities for accelerated tree breeding.
650	1	4	_aPINUS SYHESTRIS
650	1	4	_aSCOTS PINE
650	1	4	_aTISSUE CULTURE
650	1	4	_aEARLY MATURATION
650	1	4	_aFLOWERING
700	1	2	_aHaggman, H. M.
700	1	2	_aAronen, T. S.
700	1	2	_aStomp, A.-M.
856	4	0	_uhttps://drive.google.com/file/d/1FBShW8tr00a2Q-ODH7uv2jOou-qsniVQ/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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