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245 1 0 _aArabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens
490 0 _vThe Plant Journal, 48(4), p.592-605, 2006
520 3 _aPlant WRKY transcription factors are key regulatory components of plant responses to microbial infection. In addition to regulating the expression of defense-related genes, WRKY transcription factors have also been shown to regulate cross-talk between jasmonate- and salicylate-regulated disease response pathways. The two pathways mediate resistance against different types of microbial pathogens, and there are numerous reports of antagonistic interactions between them. Here we show that mutations of the Arabidopsis WRKY33 gene encoding a WRKY transcription factor cause enhanced susceptibility to the necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola concomitant with reduced expression of the jasmonateregulated plant defensin PDF1.2 gene. Ectopic over-expression of WRKY33, on the other hand, increases resistance to the two necrotrophic fungal pathogens. The wrky33 mutants do not show altered responses to a virulent strain of the bacterial pathogen seudomonas syringae, although the ectopic expression of WRKY33 results in enhanced susceptibility to this pathogen. The susceptibility of WRKY33-over-expressing plants to P. syringae is associated with reduced expression of the salicylate-regulated PR-1 gene. The WRKY33 transcript is induced in response to pathogen infection, or treatment with salicylate or the paraquat herbicide that generates activated oxygen species in exposed cells. WRKY33 is localized to the nucleus of plant cells and recognizes DNA molecules containing the TTGACC W-box sequence. Together, these results indicate that pathogen-induced WRKY33 is an important transcription factor that regulates the antagonistic relationship between defense pathways mediating responses to P. syringae and necrotrophic pathogens.
650 1 4 _aDISEASE RESISTANCE
650 1 4 _aBOTRYTIS CINEREA
650 1 4 _aALTERNARIA BRASSICICOLA
650 1 4 _aNECROTROPHE
650 1 4 _aPSEUDOMONAS SYRINGAE
650 1 4 _aWRKY33 TRANSCRIPTION FACTOR
700 1 2 _aZheng, Z.
700 1 2 _aQamar, S.A.
700 1 2 _aChen, Z.
700 1 2 _aMengiste, T.
856 4 0 _uhttps://drive.google.com/file/d/1rf9tjxIK5jUJoy31tg-J0f_H3FapvhNC/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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