000			02122nam a2200229Ia 4500
003			MX-MdCICY
005			20250625124724.0
040			_cCICY
090			_aB-8642
245	1	0	_aVisualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development
490	0		_vThe Plant Journal, 9(5), p.745-753, 1996
520	3		_aUsing a highly synchronous in vitro tuberization system, in combination with an amplified restriction fragment polymorphism (AFLpt)-derived technique for RNA fingerprinting (cDNA-AFLPt), transcriptional changes at and around the time point of potato tuberization have been analyzed. The targeted expression analysis of a specific transcript coding for the major potato storage protein, patatin and a second transcript, coding for ADP-glucose pyrophosphorylase, a key gene in the starch biosynthetic pathway is described. This paper confirms that kinetics of expression revealed by cDNA-AFLP analysis are comparable to those found in Northern analysis. Furthermore, this paper reports the isolation and analysis of two tuberspecific transcript-derived fragments (TDFs)coding for the lipoxygenase enzyme, which are differentially induced around the time point of tuber formation. Analysis of the two Iox TDFs demonstrates that it is possible to dissect the expression modalities of individual transcripts, not independently detectable by Northern analysis. Finally, it is shown that using cDNA-AFLP, rapid and simple verification of band identity may be achieved. The results indicate that cDNA-AFLP is a broadly applicable technology for identifying developmentally regulated genes.
700	1	2	_aBachem, C.W.B.
700	1	2	_avan der Hoeven, R.S.
700	1	2	_ade Bruijn, S.M.
700	1	2	_aVreugdenhil, D.
700	1	2	_aZabeau, M.
700	1	2	_aVisser, R.G.F.
856	4	0	_uhttps://drive.google.com/file/d/1NCcSS8Q4A2y8ujLyz-SJ7W0tSweim4Zo/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c42945 _d42945