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245 1 0 _aSinorhizobium meliloti nifA mutant induces different gene expression profile from wild type in Alfalfa nodules
490 0 _vCell Research, 16(10), p.818-829, 2006
520 3 _aSeveral studies have demonstrated that the Rhizobium nifA gene is an activator of nitrogen fixation acting in nodule bacteria. To understand the effects of the Sinorhizobium meliloti nifA gene on Alfalfa, the cDNA-AFLP technique was employed to study the changes in gene expression in nifA mutant nodules. Among the approximately 3,000 transcriptderived fragments, 37 had differential expression levels. These expression levels were subsequently confirmed by reverse Northern blot and RT-polymerase chain reaction. Sequence analyses revealed that 21 cDNA fragments corresponded to genes involved in signal communication, protein degradation, nutrient metabolism, cell growth and development.
650 1 4 _aCDNA-AFLP
650 1 4 _aGENE EXPRESSION
650 1 4 _aNODULE
650 1 4 _aSINORHIZOBIUM MELILOTI
650 1 4 _aNIFA
700 1 2 _aGong, Z.Y.
700 1 2 _aHe, Z.S.
700 1 2 _aZhu, J.B.
700 1 2 _aYu, G.Q.
700 1 2 _aZou. H.S.
856 4 0 _uhttps://drive.google.com/file/d/1m268xDN51th-qcmemiMUUexCy4tQLm9o/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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