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245 1 0 _aExpression of ethylene-related expansin genes in cool-stored ripening banana fruit
490 0 _vPlant Science, 170(5), p.962-967, 2006
520 3 _aExpansins are cellular proteins expressed in the course of cell wall loosening during fruit ripening. There is no information about the relationship between expansins and ripening of chilling injury (CI)-affected banana fruit. Banana fruit were pre-treated with 0 or 1000 mL/L propylene (functional ethylene analogue)for 16 h and then stored at 7 8C. CI symptoms of untreated control fruit appeared after 4 days, while propylene pre-treated fruit showed CI symptoms after 7 days. Thus, stimulation of ripening with propylene applied prior to storage at low temperature tended to alleviate CI. The fruit were stored for 8 days at 7 8C and then transferred to 22 8C, followed by treatment with 1000 mL/L propylene to initiate ripening. The propylene treatment accelerated color change, increased ethylene production rate and caused a more rap id decrease in peel and pulp firmness. Two banana expansins, AY083168 and AF539540 (GeneBank), were chosen as the target genes MaExp1 and MaExp2, respectively. RNA blotting analysis showed no accumulation of either MaExp1 or MaExp2 transcripts in banana fruit during low temperature storage. Expansin genes were expressed more intensively in propylene pre-treated fruit than in control fruit upon removal from cold storage for propylene-initiated ripening. The results suggest that increased tolerance of banana fruit pre-treated with propylene to low temperatureinduced chilling was related to higher post-storage ethylene production rates and enhanced expression of MaExp1 and MaExp2.
650 1 4 _aBANANA
650 1 4 _aCHILLING INJURY
650 1 4 _aETHYLENE
650 1 4 _aEXPANSIN
650 1 4 _aLOW TEMPERATURE
700 1 2 _aWang, Y.
700 1 2 _aLu, W.
700 1 2 _aJiang, Y.
700 1 2 _aLuo, Y.
700 1 2 _aJiang, W.
700 1 2 _aJoyce, D.
856 4 0 _uhttps://drive.google.com/file/d/1ego6-0ymgl2c5EGwwiqJlz3peFg3tbeB/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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