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| 090 | _aB-8749 | ||
| 245 | 1 | 0 | _aDilution methods to deprive Chlamydomonas reinhardtii cultures of sulfur for subsequent hydrogen photoproduction |
| 490 | 0 | _vInternational Journal of Hydrogen Energy, 27, p.1245-1249, 2002 | |
| 520 | 3 | _aSulfur deprivation of Chlamydomonas reinhardtii cultures gradually inactivates photosynthetic O2 evolution and leads to the establishment of anaerobiosis in the medium. Sulfur-deprived algal cultures kept under anaerobic conditions will then produce H2 gas for 3-5 days under continuous illumination. Currently, sulfur deprivation is achieved by mechanical centrifugation of cultures grown in sulfur-replete medium, followed by extensive and costly washing. The cells are 8nally resuspended in sulfur-free medium. The current study investigates two procedures to deprive algal cultures of sulfur that eliminate the centrifugation step. These procedures involve sulfur deprivation by dilution of sulfur-replete cultures into either sulfur-limited medium or sulfur-free medium. We demonstrate that e:cient H2 photoproduction can be achieved on a timely basis using either procedure. However, the dilution of sulfate-replete algal cultures 1:10 v=v into sulfur-free medium is the most appropriate procedure. These observations serve as the basis for developing an algal H2-production system that is cheaper, less time-consuming, and less amenable to contamination with other microorganisms than systems employing centrifugation for sulfur deprivation. | |
| 650 | 1 | 4 | _aPHOTOBIOLOGICAL HYDROGEN PRODUCTION |
| 650 | 1 | 4 | _aSULFUR DEPRIVATION |
| 650 | 1 | 4 | _aGREEN ALGAE |
| 650 | 1 | 4 | _aPHOTOBIOREACTORS |
| 700 | 1 | 2 | _aLaurinavichene, T.V. |
| 700 | 1 | 2 | _aTolstygina, I.V. |
| 700 | 1 | 2 | _aGaliulina, R.R. |
| 700 | 1 | 2 | _aGhirardi, M.L. |
| 700 | 1 | 2 | _aSeibert, M. |
| 700 | 1 | 2 | _aTsygankov, A.A. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1mwEtyxZQJS4GKBrN6UgEJi_FSQp_DpA7/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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