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090			_aB-8869
245	1	0	_aImmobilization of glucosidase onto silica-based, amino functionalized beads for enzymatic hydrolysis of urinary phenol prior to liquid chromatographic analysis
490	0		_vAnalytica Chimica Acta, 292(1-2), p.23-30, 1994
520	3		_aAn amino-functionalized silica commercial liquid chromatography packing material was used instead of controlled- pore glass as the support to react with glutaraldehyde and then to directly immobilize the /3-glucosidase. After enzymatic hydrolysis, the urinary phenol was separated by reversed-phase liquid chromatography and monitored by an electrochemical detector at 0.85 V (vs. Ag/AgCl). Factors that affect the immobilization and hydrolysis were investigated. The proposed method provides a simpler procedure with a less expensive material for glucosidase immobilization, and the electrochemical detection avoids the interference from other urinary species and hence simplifies the chromatogram obtained. The detection limit was 0.2 ng with a 28 ~1 injection. The relative standard deviations for 1.2 ng and 2.0 ng levels of phenols were 1.16
650	1	4	_aENZYMATIC METHODS
650	1	4	_aLIQUID CHROMATOGRAPHY
650	1	4	_aPACKING MATERIALS
650	1	4	_aGLUCOSIDASE IMMOBILIZATION
650	1	4	_aPHENOL
650	1	4	_aURINARY PHENOL
700	1	2	_aJen, J.F.
700	1	2	_aZen, J.H.
700	1	2	_aCheng, F.C.
700	1	2	_aYang, G.Y.
856	4	0	_uhttps://drive.google.com/file/d/1ItNie1pJVIj6OGN3WdRX0UiARS5_S5iE/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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