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090			_aB-8878
245	1	0	_aEmulsion PCR-based method to detect Y chromosome microdeletions
490	0		_vAnalytical BioChemistry, 367(2), p.173-178, 2007
520	3		_aDeletions in Y chromosome are thought to be pathologically involved in some cases of male infertility associated with azoospermia or oligozoospermia. An emulsion-based multiplex PCR method was developed for detecting Y chromosome microdeletions in infertile men and a plasma sample of pregnant women carrying a male fetus. The sensitivity of multiplex PCR in emulsion was evaluated. Conventional PCR was also carried out for comparison. A total of 13 sequence-tagged sites (STSs)distributed in the AZF region were analyzed simultaneously with this method. The SRY gene was also detected as the inner control. Results showed that Y chromosome microdeletions were found in 4 of 19 infertile patients. Also, in 1 of 63 samples collected from pregnant women, microdeletions were found in some of the detected sites. It is suggested that the emulsion PCR assay was proven to be a promising diagnostic tool and could be widely used in further clinical and academic research.
650	1	4	_aEMULSIONS
650	1	4	_aMULTIPLEX PCR
650	1	4	_aY CHROMOSOME MICRODELETIONS
700	1	2	_aGe, Q.
700	1	2	_aLiu, Z.
700	1	2	_aBai, Y.
700	1	2	_aZhang, D.
700	1	2	_aYu, P.
700	1	2	_aLu, Z.
856	4	0	_uhttps://drive.google.com/file/d/1lRYB7RBZhI5cKmK3S5wX4zmf1f2llgJu/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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