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245	1	0	_aGeneration of Leishmania donovani axenic amastigotes: their growth and biological characteristics
490	0		_vInternational Journal for Parasitology, 34(2), p.205-217, 2004
520	3		_aIn this report, we describe an in vitro culture system for the generation and propagation of axenic amastigotes from the well characterised 1S-CL2D line of Leishmania donovani. Fine structure analyses of these in vitro-grown amastigotes demonstrated that they possessed morphological features characteristic of L. donovani tissue-derived amastigotes. Further, these axenic amastigotes (LdAxAm)were shown to synthesise and release a secretory acid phosphatase isoform similar to that produced by intracellular amastigotes. Such LdAxAm also expressed surface membrane 30-nucleotidase enzyme activity similar to that of tissue-derived amastigotes. Moreover, LdAxAm, in contrast to promastigotes, expressed significant levels of the amastigote-specific A2 proteins. In addition, LdAxAm, derived from long term cultures of Ld 1S-CL2D promastigotes, had significant infectivity for both human macrophages in vitro and for hamsters in vivo. Thus, the in vitro culture system described herein provides a useful tool for the generation of large quantities of uniform populations of axenic amastigotes of the L. donovani 1S-CL2D line. The availability of such material should greatly facilitate studies concerning the cell and molecular biology of this parasite developmental stage.
650	1	4	_aTRYPANOSOMATID
650	1	4	_aLEISHMANIASIS
650	1	4	_aPARASITE
650	1	4	_aCULTURE SYSTEM
650	1	4	_aINFECTIVITY
650	1	4	_aVIRULENCE
700	1	2	_aDebrabant, A.
700	1	2	_aJoshi, M.B.
700	1	2	_aPimenta, P.F.P.
700	1	2	_aDwyer, D.M.
856	4	0	_uhttps://drive.google.com/file/d/18cfTZqP-Hd9DOWpytgkMPaYfad5mK5l0/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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