000 01724nam a2200265Ia 4500
003 MX-MdCICY
005 20250625124730.0
040 _cCICY
090 _aB-8999
245 1 0 _aUse of suppression subtractive hybridization approach to identify genes differentially expressed during early banana fruit development undergoing changes in ethylene responsiveness
490 0 _vPlant Science, 172(5), p.1025-1036, 2007
520 3 _aLittle is known about the early green developmental stage of banana fruit before the commercial harvesting stage. In this study, we demonstrate that banana fruit (cv Cavendish)grown under our pedoclimatical conditions undergoes changes in ethylene responsiveness between 40 (immature fruit unable to respond to ethylene), 60 and 90 DAF (days after flowering; early and late mature fruit able to respond to ethylene, respectively). Further, we have combined subtractive suppression hybridization (SSH)and macro-array hybridization to construct four different SSH libraries comprising a total of 3072 clones and identify 876 clones that are differentially expressed during fruit ripening. Some of these positive clones were partially sequenced to generate ESTs. Sequence analysis revealed that 163 clones on 177 (92
650 1 4 _aBANANA
650 1 4 _aRIPENING
650 1 4 _aGENE EXPRESSION
650 1 4 _aQUALITY
650 1 4 _aSSH
700 1 2 _aMbeguie-A-Mbeguie, D.
700 1 2 _aHubert, O.
700 1 2 _aSabau, X.
700 1 2 _aChillet, M.
856 4 0 _uhttps://drive.google.com/file/d/1O7p6HT1R5tymZI5qKAbUGLlIj3dfq_hT/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 _2Loc
_cREF1
008 250602s9999 xx |||||s2 |||| ||und|d
999 _c43259
_d43259