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090			_aB-9022
245	1	0	_aMethylene Blue Stainability of Root-lSp Protoplasts as an Indicator of Aluminum Tolerance in a Wide Range of Plant Species, Cultivars and Lines
490	0		_vSoil Sci. Plant Nutr., 51(7), p.991-998, 2005
520	3		_aWe previously developed a new simple technique of methylene blue (MB)staining for the discrimination of aluminum (Al)-tolerant protoplasts from 4 plant species (rice, oats, maize and pea). The objectives of the present study were to conflrm the applicability of this technique to a wider range of plant species, cultivars and lines, and to identify a common strategy for the early stage of Al tolerance. A total of 10 plant species, two rachiaria spp., two Oryza spp., buckwheat, maize, pea, triticale, wheat and barley, corresponding to 18 different plant samples (species, cultivars, and lines), were used. Al tolerance (relative net root elongation of the longest root), which was screened at 20 pM AlC13 in 0.2 mM CaC12 (pH 4.9)for 24 h, ranged widely from 10 to 88. Among cultivars and lines within the same species, Al accumulation in the root tip portion was higher in Al-sensitive plants, corresponding to more severe permeabilization of the plasma membranes (PM). Protoplasts isolated mainly from the epidermis, and outer and central parts of the cortex were stained to different degrees by MB, and the blue color was observed both on the surface and inside the protopladlts. Color pictures obtained after staining for 3 min with 0.1
650	1	4	_aALUMINUM TOLERANCE
650	1	4	_aMETHYLENE BLUE STAINABILITY
650	1	4	_aPLASMA MEMBRANE NEGATIVITY
650	1	4	_aPLASMA MEMBRANE PERMEABILITY
650	1	4	_aPROTOPLAST
700	1	2	_aWagatsuma, T.
700	1	2	_aHossain Khan, M.S.
700	1	2	_aRao, I.M.
700	1	2	_aWenzl, P.
700	1	2	_aTawaraya, K.
700	1	2	_aYamamoto, T.
700	1	2	_aKawamura, T.
700	1	2	_aKawamura, T.
700	1	2	_aIshikawa, S.
856	4	0	_uhttps://drive.google.com/file/d/1oG4E5suwCM-7vcrC6rk9xsYbvL8xY1tr/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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