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245	1	0	_aAgrobacterium-mediated transformation of Sclerotinia sclerotiorum
490	0		_vJournal of MicroBiological Methods, 65(1), p.220-207, 2006
520	3		_aAscospores from the phytopathogenic fungus Sclerotinia sclerotiorum were transformed to hygromycin B resistance by co-cultivation with Agrobacterium tumefaciens. Transformed spores germinated and grew on PDA supplemented with 100 ug/ml hygromycin B. The presence of mitotically stable hph gene integration at random sites in the genome was confirmed by PCR and Southern blot analysis. A transformation frequency of 8 × 10- 5 was achieved in five separate experiments. This study is the first report of success co-cultivating A. tumefaciens with S. sclerotiorum. This report of a reproducible Agrobacterium-mediated transformation method should allow the development of T-DNA tagging as a system for insertional mutagenesis in S. sclerotiorum and provide a simple and reliable method for genetic manipulation.
700	1	2	_aWelda, R. J.
700	1	2	_aColin, C. E.
700	1	2	_aRidgwaya, H. J.
856	4	0	_uhttps://drive.google.com/file/d/1lMPEK8erbud-SSE3qm_hK2_EguHy1yDp/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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