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245 1 0 _aPrenylation enhances cytotoxicity of apigenin and liquiritigenin in rat H4IIE hepatoma and C6 glioma cells
490 0 _vFood and Chemical Toxicology, 45(1), p.119-124, 2006
520 3 _aAntioxidative as well as cytotoxic effects of the prenylated flavonoids licoflavone C (8-prenylapigenin)and isobavachin (8-prenylliquiritigenin)were investigated in comparison to the corresponding non-prenylated flavonoids (apigenin, liquiritigenin)and vitexin (apigenin-C8-glucoside)using metabolically active H4IIE hepatoma and metabolically poorly active C6 glioma cells. None of the substances showed radical scavenging activities in the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-assay nor were they effective in protection against H2O2-induced intracellular 20,70-dichlorodihydrofluorescein (H2DCF)oxidation (fluorescent probe for oxidative stress)in H4IIE and C6 cells. When the intrinsic effects of the substances were investigated, licoflavone C and isobavachin exerted a pronounced toxicity in both H4IIE (IC50 values of 42 ± 5 and 96 ± 19 lmol/L)and C6 cells (IC50 values of 37 ± 6 and 69 ± 3 lmol/L)while the non-prenylated analogues as well as the glycosylated derivate vitexin showed almost no cytotoxic effect up to 250 lmol/L. In H4IIE cells the induction of apoptotic cell death by licoflavone C and icobavachin was detected as an activation of caspase 3/7 (6- and 3.3-fold, respectively). Based on these experiments we suggest that C8-prenylation of a flavonoid enhances the cytotoxicity inducing an apoptotic cell death in H4IIE cells without affecting antioxidative properties.
650 1 4 _aAPOPTOSIS
650 1 4 _aCYTOTOXICITY
650 1 4 _aDPPH ASSAY
650 1 4 _aOXIDATIVE STRESS
700 1 2 _aWatjen, W.
700 1 2 _aWeber, W.
700 1 2 _aLou, YJ.
700 1 2 _aWang, ZQ.
700 1 2 _aChovolou, Y.
700 1 2 _aKampkotter, A.
700 1 2 _aProksch, P.
856 4 0 _uhttps://drive.google.com/file/d/1ClvBmkLpxqOstTKHFjYcyZNTGHSQevyq/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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