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090			_aB-9220
245	1	0	_aSequence-based approach for identification of cell wall proteins in Saccharomyces cerevisiae
490	0		_vCurrent Genetics, 5(40), p.311-316, 2002
520	3		_aOpen reading frames (ORFs)in the genome of Saccharomyces cerevisiae were screened for cell wall proteins and extracellular proteins, using an in silico sequence analysis combined with biochemical examination. The selection criteria used in the sequence analysis were the presence of a signal sequence for secretion and the absence of any targeting and retention signal to/in intracellular components. By using the PSORT II program, 163 ORFs/proteins were selected as potential extracellular proteins, including cell wall proteins. Of these, 51 ORFs/proteins of unknown localization and more than 120 amino acids in size were further studied on their cellular localization. A hemagglutinin (HA)epitope was inserted in the most C-terminus of each protein and the resulting HA-tagged protein was expressed under the authentic promoter in yeast cells. Out of the 51 constructs, 35 gave protein bands on Western blots. Examination of proteins in fractionated samples identified 11 extracellular proteins; six proteins that were weakly associated with the cell wall and five proteins that were relatively tightly associated with the cell wall.
650	1	4	_aSACCHAROMYCES CEREVISIAE
650	1	4	_aEXTRACELLULAR PROTEINS
650	1	4	_aCELL WALL PROTEINS
650	1	4	_aIN SILICO SEQUENCE ANALYSIS
700	1	2	_aTerashima, H.
700	1	2	_aFukuchi, S.
700	1	2	_aNakai, K.
700	1	2	_aArisawa, M.
700	1	2	_aHamada, K.
700	1	2	_aYabuki, N.
700	1	2	_aKitada, K.
856	4	0	_uhttps://drive.google.com/file/d/1RBU51EB_2Nhm6TAV0npB0paVlilrc7j5/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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