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| 005 | 20250625140609.0 | ||
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| 245 | 1 | 0 | _aThe Arabidopsis MATE Transporter TT12 Acts as a Vacuolar Flavonoid/H1-Antiporter Active in Proanthocyanidin-Accumulating Cells of the Seed Coat |
| 490 | 0 | _vThe Plant Cell, 19(6), p.2023-2038, 2007 | |
| 520 | 3 | _aPhenotypic characterization of the Arabidopsis thaliana transparent testa12 (tt12)mutant encoding a membrane protein of the multidrug and toxic efflux transporter family, suggested that TT12 is involved in the vacuolar accumulation of proanthocyanidin precursors in the seed. Metabolite analysis in tt12 seeds reveals an absence of flavan-3-ols and proanthocyanidins together with a reduction of the major flavonol quercetin-3-O-rhamnoside. The TT12 promoter is active in cells synthesizing proanthocyanidins. Using translational fusions between TT12 and green fluorescent protein, it is demonstrated that this transporter localizes to the tonoplast. Yeast vesicles expressing TT12 can transport the anthocyanin cyanidin-3-O-glucoside in the presence ofMgATP but not the aglycones cyanidin and epicatechin. Inhibitor studies demonstrate that TT12 acts in vitro as a cyanidin-3-O-glucoside/Hþ-antiporter. TT12 does not transport glycosylated flavonols and procyanidin dimers, and a direct transport activity for catechin-3-O-glucoside, a glucosylated flavan-3-ol,was not detectable. However, catechin-3-O-glucoside inhibited TT12-mediated transport of cyanidin-3-O-glucoside in a dose-dependent manner, while flavan-3-ol aglycones and glycosylated flavonols had no effect on anthocyanin transport. It is proposed that TT12 transports glycosylated flavan-3-ols in vivo.Mutantbanyuls (ban)seeds accumulateanthocyaninsinsteadofproanthocyanidins,yet the ban tt12doublemutantexhibits reduced anthocyanin accumulation, which supports the transport data suggesting that TT12 mediates anthocyanin transport in vitro. | |
| 700 | 1 | 2 | _aMarinova, K. |
| 700 | 1 | 2 | _aPourcel, L. |
| 700 | 1 | 2 | _aWeder, B. |
| 700 | 1 | 2 | _aSchwarz, M. |
| 700 | 1 | 2 | _aBarron, D. |
| 700 | 1 | 2 | _aRoutaboul, J.C. |
| 700 | 1 | 2 | _aDebeaujon, I. |
| 700 | 1 | 2 | _aDebeaujon, I. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/14AXobXtxFgXXrfmqsBgmYuUfWBqbpw0A/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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