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| 245 | 1 | 0 | _aMaize phytoene desaturase and z-carotene desaturase catalyse a poly-Z desaturation pathway: implications for genetic engineering of carotenoid content among cereal crops |
| 490 | 0 | _vJournal of Experimental Botany, 54(391), p.2215-2230, 2003 | |
| 520 | 3 | _aCarotene desaturation, an essential step in the biosynthesis of coloured carotenoids, has received much attention (1)as a target of bleaching herbicide action, (2)as a determinant of geometric isomer states of carotenoids and their metabolites, and (3)as a key modulator of accumulation and structural variability of carotenoids. Having previously isolated and functionally characterized the cDNA encoding the ®rst enzyme in maize carotene desaturation, phytoene desaturase (PDS), the isolation and functional characterization of the second desaturase, a maize endosperm cDNA (2265 bp)encoding zetacarotene (z-carotene)desaturase (ZDS)is reported here. Functional analysis of the concerted actions of maize PDS and ZDS ex situ showed these enzymes to mediate a poly-Z desaturation pathway to the predominate geometric isomer ,9,7¢,9¢-tetra-Z-lycopene (poly-Zlycopene or prolycopene), and not the all-trans substrate required of the downstream lycopene cyclase enzymes. This ®nding suggests a rate-controlling isomerase associated with the carotene desaturases as a corollary of a default poly-Z carotenoid biosynthetic pathway active in planta for maize. Comparative gene analysis between maize and rice revealed that genes encoding PDS and ZDS are single copy; the Zds cDNA characterized here was mapped to maize chromosome 7S and vp9 is suggested as a candidate locus for the structural gene while y9 is ruled out. Classical genetic resources were used to dissect the desaturation steps further and hydroxyphenylpyruvate dioxygenase was linked to the vp2 locus, narrowing candidate loci for an obligate isomerase in maize to only a few. Since the ®rst functional analysis of the paired carotene desaturases for a cereal crop is reported here, the implications for the genetic modi®cation of the provitamin A content in cereal crops such as rice and maize, are discussed. | |
| 650 | 1 | 4 | _aBIOSYNTHESIS |
| 650 | 1 | 4 | _aCAROTENOID |
| 650 | 1 | 4 | _aDESATURASE |
| 650 | 1 | 4 | _aGENES |
| 650 | 1 | 4 | _aISOPRENOID |
| 650 | 1 | 4 | _aMAIZE |
| 650 | 1 | 4 | _aMUTANTS |
| 650 | 1 | 4 | _aPHYTOENE |
| 650 | 1 | 4 | _aRICE |
| 650 | 1 | 4 | _aZETACAROTENE |
| 700 | 1 | 2 | _aMatthews, P.D. |
| 700 | 1 | 2 | _aLuo, R. |
| 700 | 1 | 2 | _aWurtzel, E.T. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1Kdw5MdzQZDqZ7TK9HuiScBWT_9E7O0ps/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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