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| 003 | MX-MdCICY | ||
| 005 | 20250625140611.0 | ||
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| 090 | _aB-9368 | ||
| 245 | 1 | 0 | _aIsolation and characterisation of Gaeumannomyces graminis var. graminis melanin mutants |
| 490 | 0 | _vMycological Research, 103(1), p.99-11, 1999 | |
| 520 | 3 | _aGaeumannomyces graminis var. graminis produces 1,8-dihydroxynaphthalene (DHN)melanin in its hyphal and hyphopodial cell walls. We isolated G. graminis mutants that were affected in their melanin biosynthesis. One was unable to synthesize DHN-melanin and, because it accumulated 2-hydroxyjuglone, a DHN melanin pathway shunt product, it is most likely to be defective in the reductase that catalyzes the conversion of 1,3,8-trihydroxynaphthalene to vermelone, the penultimate reaction in DHN synthesis. Genetic crosses with our wild-type strain indicated that this trihydroxynaphthalene reductase de®ciency was the result of a single mutation. Another mutant constitutively synthesized DHN melanin and genetic crosses with our wild-type strain suggested that this heavily melanized mutant had a single mutation responsible for its phenotype. This mutant produced more melanin than the wild-type strain as measured by Azure A binding to melanin. The wild type and constitutively melanized mutant hyphae were more hydrophobic and more resistant to lytic enzymes, benomyl, restrictive temperature, and uv light than the non-melanized mutant, which also autolysed more readily. The non-melanized mutant was not more sensitive to heavy metal than the melanized strains. In addition, the non-melanized mutant was unaltered in pathogenicity to rice, whereas the constitutively melanized mutant was ess pathogenic. The constitutively melanized mutant produced less extracellular lytic enzymes than the wild-type and the non-melanized mutant, which may explain its reduced virulence. | |
| 700 | 1 | 2 | _aFrederick, B.A. |
| 700 | 1 | 2 | _aCaesar-Tonthat, T.-C. |
| 700 | 1 | 2 | _aWheeler, M. H. |
| 700 | 1 | 2 | _aSheehan, K. B. |
| 700 | 1 | 2 | _aEdens, W. A. |
| 700 | 1 | 2 | _aHenson, J. M. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1YqQbdjj0fWuA6qaRSHGYElrDIfs15ds4/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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