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245 1 0 _aDroplet vitrification of apical meristems: a cryopreservation protocol applicable to all Musaceae
490 0 _vPlant Science, 168(1), p.45-55, 2005
520 3 _aOne millimeter sized shoot-tips excised from rooted in vitro plants of the genera Musa and Ensete were successfully cryopreserved with the droplet vitrification technique.We show that the loading phase can be prolonged up to 7 h and that the optimal length of PVS2 treatment is 30-50 min at 0 8C. Ultra rapid freezing and thawing rates proved to be essential for high and reproducible post-thaw regeneration rates. This results in an increase of 23-46
650 1 4 _aBANANA
650 1 4 _aCRYOPRESERVATION
650 1 4 _aGERMPLASM COLLECTION
650 1 4 _aMERISTEM CULTURE
650 1 4 _aMUSA
650 1 4 _aVITRIFICATION
700 1 2 _aPanis, B.
700 1 2 _aPiette, B
700 1 2 _aSwennen, R.
856 4 0 _uhttps://drive.google.com/file/d/1F8debiqSVUYWhJ5eqlIVCnh0mg78mIhh/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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