000			02287nam a2200289Ia 4500
003			MX-MdCICY
005			20250625140611.0
040			_cCICY
090			_aB-9394
245	1	0	_aIncreased expression of transgene in stably transformed cells of Dunaliella salina by matrix attachment regions
490	0		_vAppl Microbiol Biotechnol, 76, p.651-657, 2007
520	3		_aNuclear matrix attachment regions (MARs)are known to bind specifically to the nuclear scaffold and are thought to influence expression of the transgenes. In our previous studies, a new deoxyribonucleic acid fragment isolated from Dunaliella salina could bind to the nuclear matrix in vitro and had the typical characteristics of MARs. In this study, to investigate effects of MARs on expression of transgenes in the stably transformed cells of D. salina, expression vectors with and without MARs, which contained chloramphenicol acetyltransferase (CAT)reporter gene driven by D. salina ribulose 1,5-bisphosphate carboxylase/ oxygenase promoter, were constructed and delivered, respectively, into cells of D. salina by electroporation. Twenty stably transformed colonies of D. salina were randomly picked out, and CAT gene expression was assayed. The results showed that the CAT enzyme of the colonies of D. salina transformed with the expression vector containing MARs averaged out about 4.5-fold higher than those without MARs, while the transgene expression variation among individuals of transformants decreased threefold. The CAT enzyme in the stably transformed lines was not significantly proportional to the gene copy numbers, suggesting that the effects of MARs on ransgene expression may not be through increasing the transgene copy numbers
650	1	4	_aCHLORAMPHENICOL ACETYLTRANSFERASE
650	1	4	_aDUNALIELLA SALINA
650	1	4	_aMATRIX ATTACHMENT REGION
650	1	4	_aTRANSGENE EXPRESSION
700	1	2	_aWang, T.
700	1	2	_aXue, L.
700	1	2	_aHou, W.
700	1	2	_aYang, B.
700	1	2	_aChai, Y.
700	1	2	_aJi, X.
700	1	2	_aWang, Y.
856	4	0	_uhttps://drive.google.com/file/d/1SwZ30Mc0CGOKAslAupoOR4VranY9tUDa/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c43644 _d43644