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090			_aB-9398
245	1	0	_aExpression of Foreign Genes in Dunaliella by Electroporation
490	0		_vMolecular Biotechnology, 30, p.185-192, 2005
520	3		_aAn electroporation procedure has been described for introducing plasmid DNA into Dunaliella salina cells. By this procedure, a bulk of plasmid DNA was delivered into the cells and retained for at least 3 d. Reverse transcriptase polymerase chain reaction (RT-PCR)and sequencing analyses indicated that the transcription and pre-mRNA splicing of ble gene (contributing the Zeocin resistance)were detected in the cells as early as 1 h after the electroporation. Individual colonies could retain the resistance to 10 mg/L Zeocin for at least 6 mo. Subsequent Southern blot analysis showed the existence of introduced plasmid DNA inside these colonies. However, most of the cells (approx 90
650	1	4	_aDUNALIELLA SALINA
650	1	4	_aELECTROPORATION
650	1	4	_aBLE GENE
650	1	4	_aZEOCIN
650	1	4	_aEPISOMAL DNA
700	1	2	_aSun, Y.
700	1	2	_aYang, Z.
700	1	2	_aGao, X.
700	1	2	_aLi, Q.
700	1	2	_aZhang, Q.
700	1	2	_aXu, Z.
856	4	0	_uhttps://drive.google.com/file/d/1GZeWNNZwIMb_zvc9WZOwT2XYQEVBznbs/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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