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245 1 0 _aIdentification of Betalains from Yellow Beet (Beta vulgaris L.)and Cactus Pear [Opuntia ficus-indica (L.)Mill.]by High-Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry
490 0 _vJ. Agric. Food Chem., 50, p.2302-2307, 2002
520 3 _aBetaxanthins, the yellow-orange water-soluble pigments from yellow beet (Beta vulgaris ssp. vulgaris cv. Bejo Zaden)and cactus pear (Opuntia ficus-indica cv. Gialla)have been investigated using an HPLC system compatible with mass spectrometry. Five novel betaxanthins were found and characterized as the immonium adducts of betalamic acid with serine, รง-aminobutyric acid, valine, isoleucine, and phenylalanine. To enable concentration of betalain samples, desalting was performed by solid-phase extraction. With this technique, betacyanins could be separated from the betaxanthins using the pH-dependent retention characteristics of red and yellow betalains. The betaxanthin fraction was taken for the preparation of betalamic acid as a precursor for semisynthetic standards. The HPLC method was applied to yellow beet and cactus pear, revealing a more complex betalain profile than described earlier, thus proving its suitability for screening of betaxanthin-containing plants as potential sources for natural food colors.
650 1 4 _aBETALAINS
650 1 4 _aBETAXANTHINS
650 1 4 _aBETACYANINS
650 1 4 _aAMINO COMPOUNDS
650 1 4 _aBETA VULGARIS
650 1 4 _aYELLOW BEET
650 1 4 _aOPUNTIA FICUS-INDICA
650 1 4 _aCACTUS PEAR
650 1 4 _aSOLID-PHASE EXTRACTION
650 1 4 _aHPLC
650 1 4 _aMASS SPECTROMETRY
700 1 2 _aStintzing, F.C.
700 1 2 _aSchieber, A.
700 1 2 _aCarle, R.
856 4 0 _uhttps://drive.google.com/file/d/1FOJ2FtFZWa92-4_Wqub6VnLFaFtVy5YF/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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