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245 1 0 _aSome AFLP amplicons are highly conserved DNA sequences mapping to the same linkage groups in two F2 populations of carrot
490 0 _vGenetics and Molecular Biology, 25, p.195-201, 2002
520 3 _aAmplified fragment length polymorphism (AFLP)is a fast and reliable tool to generate a large number of DNA markers. In two unrelated F2 populations of carrot (Daucus carota L.), Brasilia x HCM and B493 x QAL (wild carrot), it was hypothesized that DNA 1)digested with the same restriction endonuclease enzymes and amplified with the same primer combination and 2)sharing the same position in polyacrylamide gels should be conserved sequences. To test this hypothesis AFLP fragments from polyacrylamide gels were eluted, reamplified, separated in agarose gels, purified, cloned and sequenced. Among thirty-one paired fragments from each F2 population, twenty-six had identity greater than 91
650 1 4 _aDAUCUS CAROTA
650 1 4 _aEVOLUTION
650 1 4 _aMOLECULAR MARKERS
650 1 4 _aSCAR
700 1 2 _aSantos, C:A.F:
700 1 2 _aSimon, F.W.
856 4 0 _uhttps://drive.google.com/file/d/1cPu-s0trNPVFp7Kt61jGHDSWXPMCulQ5/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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