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245	1	0	_aYeast communities in a natural tequila fermentation
490	0		_vAntonie van Leeuwenhoek, 68(2), p.151-160, 1995
520	3		_aFresh and cooked agave, Drosophila spp., processing equipment, agave molasses, agave extract, and fermenting must at a traditional tequila distillery (Herradura, Amatitan, Jalisco, Mrxico)were studied to gain insight on the origin of yeasts involved in a natural tequila fermentations. Five yeast communities were identified. (1)Fresh agave contained a diverse mycobiota dominated by Clavispora lusitaniae and an endemic species, Metschnikowia agaveae. (2)Drosophila spp. from around or inside the distillery yielded typical fruit yeasts, in particular Hanseniaspora spp., Pichia kluyveri, and Candida krusei. (3)Schizosaccharomyces pombe prevailed in molasses. (4)Cooked agave and extract had a considerable diversity of species, but included Saccharomyces cerevisiae. (5)Fermenting juice underwent a gradual reduction in yeast heterogeneity. Torulaspora delbrueckii, Kluyveromyces marxianus, and Hanseniaspora spp. progressively ceded the way to S. cerevisiae, Zygosaccharomyces bailii, Candida milleri, and Brettanomyces spp. With the exception of Pichia membranaefaciens, which was shared by all communities, little overlap existed. That separation was even more manifest when species were divided into distinguishable biotypes based on morphology or physiology. It is concluded that crushing equipment and must holding tanks are the main source of significant inoculum for the fermentation process. Drosophila species appear to serve as internal vectors. Proximity to fruit trees probably contributes to maintaining a substantial Drosophila community, but the yeasts found in the distillery exhibit very little similarity to those found in adjacent vegetation. Interactions involving killer toxins had no apparent direct effects on the yeast community structure.
650	1	4	_aTEQUILA
650	1	4	_aFERMENTATION
650	1	4	_aAGAVE
650	1	4	_aYEAST COMMUNITY
650	1	4	_aDROSOPHILA
700	1	2	_aLachance, M.
856	4	0	_uhttps://drive.google.com/file/d/11WFHpP5-UTDzG3DlDPA9cGeDEHHTy1PZ/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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