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| 003 | MX-MdCICY | ||
| 005 | 20250625140614.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-9548 | ||
| 245 | 1 | 0 | _aEnzymatic Synthesis and Purification of Aromatic Coenzyme A Esters |
| 490 | 0 | _vAnalytical BioChemistry, 302(2), p.305-312, 2002 | |
| 520 | 3 | _aTwo recombinant His-tagged proteins, a plant 4-coumarate: coenzyme A ligase (EC 6.2.1.12)and a bacterial benzoate:coenzyme A ligase (EC 6.2.1.25), were expressed in Escherichia coli and purified in a single step using Ni-chelating chromatography. Purified enzymes were used to synthesize cinnamoyl-coenzyme A (CoA), p-coumaroyl-CoA, feruloyl-CoA, caffeoyl-CoA, and benzoyl-CoA. Conversions up to 95 | |
| 650 | 1 | 4 | _aCOENZYME A |
| 650 | 1 | 4 | _aELECTROSPRAY IONIZATION-MASS SPECTROMETRY |
| 650 | 1 | 4 | _aAFFINITY CHROMATOGRAPHY |
| 650 | 1 | 4 | _aSOLIDPHASE EXTRACTION |
| 650 | 1 | 4 | _a4-COUMARATE |
| 650 | 1 | 4 | _aCOA LIGASE |
| 650 | 1 | 4 | _aBENZOATE |
| 650 | 1 | 4 | _aCOA LIGASE |
| 650 | 1 | 4 | _aRECOMBINANT PROTEINS |
| 700 | 1 | 2 | _aBeuerle, T. |
| 700 | 1 | 2 | _aPichersky, E. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/open?id=1xLxsFGbOdldkk0f1u5wYqNSORLCCrbmq&usp=drive_copy _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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