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090			_aB-9593
245	1	0	_aA one-step method to convert vectors into binary vectors suited for Agrobacterium -mediated transformation
490	0		_vCurrent Genetics, 45(4), p.242-248, 2004
520	3		_aBacterial artificial chromosomes (BACs)are widely used for the construction of physical maps, positional-cloning and whole-genome sequencing strategies. Unfortunately, their use for functional genomics is limited, as currently there is no efficient method to use BACs directly for complementation. We describe a novel strategy for one-step conversion of any BAC into a binary BAC (BIBAC). Using Agrobacterium umefaciens, these BIBACs can be efficiently transformed to virtually all organisms, including plants, fungi, yeasts and human cells. As the strategy is based on in vivo recombineering and does not depend on restriction sites, it is applicable to any vector. To show the feasibility of the method five BACs, containing 0-75 kb of fungal DNA, were converted into BIBACs. These were subsequently transformed to the plant pathogenic fungus Fusarium oxysporum f.sp. lycopersici and to Aspergillus awamori, a filamentous fungus often used for large-scale protein production. Molecular characterisation of the transformants showed that the BIBACs were efficiently transferred to the fungi and stably integrated into their genomes.
650	1	4	_aBINARY VECTOR
650	1	4	_aBACTERIAL ARTIFICIAL
650	1	4	_aCHROMOSOME
650	1	4	_aBIBAC
650	1	4	_aRECOMBINEERING
700	1	2	_aTakken, F.L.W.
700	1	2	_aVan Wijk, R.
700	1	2	_aMichielse, C.B.
700	1	2	_aHouterman, P.M.
700	1	2	_aRam, A.F.J.
700	1	2	_aCornelissen, B.J.C.
856	4	0	_uhttps://drive.google.com/file/d/1irTNIcA51dUsJr-duRe3sdqsC5IgWEjj/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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