000			02031nam a2200289Ia 4500
003			MX-MdCICY
005			20250625140615.0
040			_cCICY
090			_aB-9605
245	1	0	_aNitrate reductase and nitrite reductase as targets to study gene silencing phenomena in transgenic plants
490	0		_vEuphytica, 93(2), p.195-200, 1997
520	3		_aNitrate assimilation is a fundamental function in any plant including those that can fix atmospheric nitrogen in symbiosis with soil bacteria. In recent years, attempts have been made to understand the biological significance of the complex regulation of this pathway using genetic engineering techniques. Transgenic plants that either over- or under-express genes of the nitrate assimilation pathway were created in order to determine whether such directed changes affect the regulation of the metabolism. Apart from interesting physiological results, unexpected gene silencing phenomena have been observed resulting from the introduction of five different transgenes derived from either the tobacco Nia or Nii genes encoding nitrate reductase and nitrite reductase, respectively. In this review, each of these five silencing phenomena is described, with the emphasis on the advantages provided by the use of both Nia and Nii genes to analyze the molecular and genetic basis of gene silencing in transgenic plants.
650	1	4	_aGUS-GLUCURONIDASE PROTEIN
650	1	4	_aNIA-NITRATE REDUCTASE GENE
650	1	4	_aNII-NITRITE REDUCTASE GENE
650	1	4	_aNIR-NITRITE REDUCTASE PROTEIN
650	1	4	_aNPT-NEOMYCIN PHOSPHOTRANSFERASE GENE
650	1	4	_aNR-NITRATE REDUCTASE PROTEIN
650	1	4	_aUIDA-GLUCURONIDASE GENE
700	1	2	_aVaucheret, E.
700	1	2	_aPalauqui, J.E.
700	1	2	_aMourrain, P.
700	1	2	_aElmayan, T.
856	4	0	_uhttps://drive.google.com/file/d/1yuRfcxjvqAtFT8Xa9hPCxuxiVhJjbz8D/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c43852 _d43852