000			01642nam a2200253Ia 4500
003			MX-MdCICY
005			20250625140616.0
040			_cCICY
090			_aB-9629
245	1	0	_aThe secretome of Pleurotus sapidus
490	0		_vProteomics, 5(18), p.4832-4838, 2005
520	3		_aDue to their unique capability to attack lignified biopolymers, extracellular enzymes of white-rot fungi enjoy an increasing interest in various fields of white biotechnology. The edible fungus Pleurotus sapidus was selected as a model organism for the analysis of the secretome by means of 2-DE. For enzyme production, the fungus was grown in submerged cultures either on peanut shells or on glass wool as a carrier material. Identification of the secreted enzymes was performed by tryptic digestion, ESI-MS/MS ab initio sequencing, and homology searches against public databases. The spectrum of secreted enzymes comprised various types of hydrolases and lignolytic enzymes of the manganese peroxidase/versatile peroxidase family. While peptidases were secreted mainly by the cultures grown on peanut shells, versatile peroxidase type enzymes dominated in the cultures grown on glass wool.
650	1	4	_a2-D ELECTROPHORESIS
650	1	4	_aBASIDIOMYCETE
650	1	4	_aEXTRACELLULAR ENZYMES
650	1	4	_aFUNGI
700	1	2	_aZorn, H.
700	1	2	_aPeters, T.
700	1	2	_aNimtz, M.
700	1	2	_aBerger, R.G.
856	4	0	_uhttps://drive.google.com/file/d/1wqz_D12qCmMl2KKfmnv1mOGEPXWNoopT/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c43876 _d43876