| 000 | 01437nam a2200265Ia 4500 | ||
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| 003 | MX-MdCICY | ||
| 005 | 20250625140616.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-9648 | ||
| 245 | 1 | 0 | _aA Simple Silica-based Method for Metagenomic DNA Extraction from Soil and Sediments |
| 490 | 0 | _vMolecular Biotechnology, 40(1), p.13-17, 2008 | |
| 520 | 3 | _aA new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. Highquality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE with Firmicutes and lactic acid bacteria-specific primers the extracted metagenomic DNA was shown to contain a mixture of bacterial genomes. This method can be used for screening bacterial diversity in soil and sediment samples | |
| 650 | 1 | 4 | _aMETAGENOMIC DNA |
| 650 | 1 | 4 | _aMICROBIAL COMMUNITY |
| 650 | 1 | 4 | _aSEDIMENTS |
| 650 | 1 | 4 | _aSOILS |
| 650 | 1 | 4 | _aDGGE |
| 700 | 1 | 2 | _aRojas-Herrera, R. |
| 700 | 1 | 2 | _aNarváez-Zapata, J. |
| 700 | 1 | 2 | _aZamudio-Maya, M. |
| 700 | 1 | 2 | _aMena-Martínez, M.E. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1uGN7ygfoHLFfEf6Vcm_zY69fIX3Tt5Ex/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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