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090 _aB-9656
245 1 0 _aCloning and characterization of a stearoyl-ACP desaturase gene from Jatropha curcas
490 0 _vJournal of Shanghai University, 11, p.182-188, 2007
520 3 _aUsing degenerate primers and RT-PCR, RACE techniques, a 1491 bp cDNA segment of stearoyl-acyl carrier protein desaturase (SAD)is cloned from developing seeds of Jatropha curcas L. The segment contains a 1191 bp of complete open reading frame (ORF). Analysis in the BLAST on NCBI shows that Jatropha curcas SAD (JSAD)gene encodes a protein precursor composed of a signal peptide of 33 amino acids and a mature peptide of 363 amino acids. The homological analysis shows that JSAD has high level of homology both in nucleotide sequence and in amino acid sequence to other plants SADs. The nucleotide and peptide identity of JSAD to Ricinus communis SAD (RSAD)is up to 89
650 1 4 _aJATROPHA CURCAS
650 1 4 _aSTEAROYL-ACYL CARRIER PROTEIN DESATURASE
650 1 4 _aFATTY ACID
650 1 4 _aGENE CLONING
650 1 4 _aBIOINFORMATICS ANALYSIS
700 1 2 _aLuo, T.
700 1 2 _aMa, D.W.
700 1 2 _aXu, Y.
700 1 2 _aDeng, W.Y.
700 1 2 _aXiao M.
700 1 2 _aQing R.W.
700 1 2 _aChen, F.
856 4 0 _uhttps://drive.google.com/file/d/1JhFuZQikpKJcMn360CYozRr31Mgiqt5J/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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