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245 1 0 _aApolar chromatography on Sephadex LH-20 combined with high-speed counter-current chromatography High yield strategy for structurally closely related analytes-Destruxin derivatives from Metarhizium anisopliae as a case study
490 0 _vJournal of Chromatography A, 1117(1), p.67-73, 2006
520 3 _aA novel high yield isolation procedure for lipophilic cyclic peptide derivatives is presented. Destruxin (dtx)A, B, D, E, and E-diol retrieval from Metarhizium anisopliae culture broth was achieved with a three-step purification protocol. After liquid-liquid extraction column chromatography over Sephadex LH-20 served as enrichment step. High-speed counter-current chromatography (HSCCC)was used for the final purification.Within the first chromatographic step dtx D and dtx E-diol were separated in purities exceeding 90
650 1 4 _aMETARHIZIUM ANISOPLIAE
650 1 4 _aDESTRUXINS
650 1 4 _aSEPHADEX-LH20
650 1 4 _aHSCCC
650 1 4 _aHIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY
700 1 2 _aSeger, C.
700 1 2 _aEberhart, K.
700 1 2 _aSturm, S.
700 1 2 _aStrasser, H.
700 1 2 _aStuppner, H.
856 4 0 _uhttps://drive.google.com/file/d/1kS_2wsFNxA-aSBntOAfZ2BlVnGmu5BbH/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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