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| 003 | MX-MdCICY | ||
| 005 | 20250625140619.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-9793 | ||
| 245 | 1 | 0 | _aExpression of a Foreign Gene in Chlamydomonas reinhardtii Chloroplast |
| 490 | 0 | _vJournal of BioScience and BioEngineering, 87(3), p.307-314, 1999 | |
| 520 | 3 | _aChimeric genes for expression of a foreign gene in the Chlamydomonas reinhardtii chloroplast were constructed. These chimeric genes are composed of the promoter from chloroplast genes, rbcL, psbA, and atpA, S- and S/-untranslated regions, and the Escherichia coli ,9-glucuronidase (GUS)structural gene (uidA)as a foreign gene. Three types of chloroplast transformants (RG, PG, and AG), which contained the rbcL-uidA, psbA-uidA, and atpA-uidA chimerlc genes integrated in the chloroplast genome, were generated by particle bombardment. The AG transformant grown under photoautotrophlc conditions showed the highest GUS activity (130 nmol/min/mg protein)so far reported in C. reinhurdtii, and the accumulated GUS protein accounted for 0.08 | |
| 650 | 1 | 4 | _aCHLAMYDOMONAS REINHARDTII |
| 650 | 1 | 4 | _aCHLOROPLAST |
| 650 | 1 | 4 | _aTRANSFORMATION |
| 650 | 1 | 4 | _aPROMOTER |
| 650 | 1 | 4 | _aEXPRESSION |
| 650 | 1 | 4 | _aP-GLUCURONIDASE |
| 700 | 1 | 2 | _aIshikura, K. |
| 700 | 1 | 2 | _aTakaoka, Y. |
| 700 | 1 | 2 | _aKato, K. |
| 700 | 1 | 2 | _aSekine, M. |
| 700 | 1 | 2 | _aYoshida, K. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/132OEbRdj6o33yG9wFEFXjOmbC5kSJtIs/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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