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245 1 0 _aDevelopment and Validation of an HPLC Method for the Characterization and Assay of the Saponins from Ilex paraguariensis A. St.-Hil (Mate)Fruits
490 0 _vJournal of Liquid Chromatography & Related Technologies, 30(1), p.87-95, 2007
520 3 _aTwo improved HPLC methods for the characterization and assay of the saponin content in green fruits of Ilex paraguariensis (erva-mate)are described. Both HPLC methods consisted of isocratic separation at room temperature, using a RP-18 column with UV-Vis detection. The first system was intended for the characterization of the main I. paraguariensis saponin fraction, while the second one was specifically developed and validated for its quantitation. Matesaponin-3 was used as external standard. From the freeze dried extract, ten peaks were characterized as saponins by TLC and HPLC analysis. The matesaponin-3 content was 5.13 g
650 1 4 _aILEX PARAGUARIENSIS
650 1 4 _aFRUITS
650 1 4 _aSAPONINS
650 1 4 _aHPLC ASSAY
650 1 4 _aVALIDATION
700 1 2 _aPavei, C.
700 1 2 _aGuzatto, P.
700 1 2 _aRos Petrovick, P.
700 1 2 _aGosmann, G.
700 1 2 _aGonzález-Ortega, G.
856 4 0 _uhttps://drive.google.com/file/d/1mu6BQfGbt9BZyE0CHe3LlkhQOFi5dW5S/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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