000			02186nam a2200289Ia 4500
003			MX-MdCICY
005			20250625140621.0
040			_cCICY
090			_aB-9888
245	1	0	_aMultiple Actin-Related Proteins of Saccharomyces cerevisiae Are Present in the Nucleus1
490	0		_vJ. Biochem., 128(4), p.665-671, 2000
520	3		_aAn increasing number of actin-related proteins (Arps), which share the basal structure with skeletal actin but possess distinct functions, have been found in a wide variety of organisms. Individual Arps of Saccharomyces cerevisiae were classified into Arps 1-10 based on the relatedness of their sequences and functions, where Arpl is the most similar to actin, and ArplO is the least similar. While Arps 1-3 and their orthologs in other organisms are localized exclusively in the cytoplasm, Arp4 (also known as Act3)is localized in the nucleus and is involved in transcriptional regulation. Here we examined the more divergent Arps for possible nuclear functions. We show that Arps 5-8 are localized in the nucleus, but ArplO is not. The nuclear export signals identified in actin are well conserved in the cytoplasmic Arps, Arps 1-3, but less conserved in the nuclear Arps. Gel filtration chromatography experiments show that the nuclear Arps are larger than monomer in size and thus are present in multi-protein complexes. Since nuclear protein complexes containing Arps are found to be responsible for histone acetylation and chromatin remodeling, it is suggested that most of the divergent Arps are involved in the transcriptional regulation through chromatin modulation.
650	1	4	_aACTIN-RELATED PROTEIN
650	1	4	_aCHROMATIN
650	1	4	_aNUCLEAR LOCALIZATION
650	1	4	_aPROTEIN COMPLEX
650	1	4	_aTRANSCRIPTIONAL REGULATION
700	1	2	_aHarata, M.
700	1	2	_aOma, Y.
700	1	2	_aTabuchi, T.
700	1	2	_aZhang, Y.
700	1	2	_aStillman, D.J.
700	1	2	_aMizuno, S.
856	4	0	_uhttps://drive.google.com/file/d/1Wz5je_k_06P_nyBoxeketn1KwL59Fh0M/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c44133 _d44133