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040			_cCICY
090			_aB-9923
245	1	0	_aThe development of an in vitro screening strategy for topically applied products
490	0		_vJournal of EthnoPharmacology, 89(1), p.81-90, 2003
520	3		_aThe objective of this study was the pharmaco-toxicological understanding of the constituents of an authenticated herbal mixture. The mixture was prepared by maceration in ethanol and subsequent dilution to produce a topically applied lotion, for which the intended target conditions are psoriasis and eczema. A three-tiered in vitro screening strategy was adopted for evaluating this product, comprising cytotoxicity assays; mutagenicity screening and therapeutic evaluation. Viability assays performed with dilutions of both the herbal concentrate and final product on organotypic cell lines indicated that neither preparation acted as an irritant. Genotoxicity screening using six strains of Salmonella typhimurium showed no mutagenic potential, and furthermore significant anti-microbial activity was evident. Therapeutic evaluation involved assessing the antioxidant potential of the extract, which can be correlated to an anti-inflammatory effect. Nitroblue-tetrazolium (NBT)assay results indicate that the extract can reduce superoxide anion generation by 45
650	1	4	_aDISMUTATION
650	1	4	_aSUPEROXIDE ANION
650	1	4	_aPROSTAGLANDIN E2
650	1	4	_aNITROBLUE-TETRAZOLIUM ASSAY
650	1	4	_a3-D SKIN MODEL
700	1	2	_aHoran, I.
700	1	2	_aClotworthy, M.
700	1	2	_aFokunang, C.N.
700	1	2	_aTomkins, P.T.
856	4	0	_uhttps://drive.google.com/file/d/1vYr6OEx0kA-DPZHDQaGvvef2lkYtDJ0V/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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