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090 _aB-9940
245 1 0 _aElectroporation of protoplasts
490 0 _vJournal of Tissue Culture Methods, 12(4), p.121-126, 1989
520 3 _aA protocol is described for the delivery- of plasmid DNA into plant protoplasts by means of electroporation. Expression of the DNA is monitored 24 h later by a "transient assay" for chloramphenicol acetyltransferase (CATL Stable transformants are recovered by selecting for kanamycin resistance using a modified version of the agarose-bead technique.
650 1 4 _aDIRECT GENE TRANSFER
650 1 4 _aSTABLE TRANSFORMATION
650 1 4 _aDNA UPTAKE
650 1 4 _aTRANSIENT EXPRESSION
650 1 4 _aCAT EXPRESSION
650 1 4 _aKANAMYCIN RESISTANCE
700 1 2 _aBates, G.W.
856 4 0 _uhttps://drive.google.com/file/d/1A_tKEEkhQO5C3gQCFaPwy9R3bITrF3_J/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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