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245 1 0 _aEfficient microRNA capture and bar-coding via enzymatic oligonucleotide adenylation
490 0 _vNature Methods, 5(9), p.777-779, 2008
520 3 _aHere we report a highly efficient and simplified strategy to preadenylate bar-coded oligonucleotides designed for microRNA (miRNA)capture and multiplex analysis. Using this approach, we enzymatically preadenylated bar-coded oligonucleotides with high efficiency when compared to the chemical method currently used by miRNA investigators. As a case study, we used these oligonucleotides in an ATP-independent ligation to miRNAs, suggesting the utility of our method in end-capture protocols and high-throughput sequencing applications.
700 1 2 _aVigneault, F.
700 1 2 _aMichael Sismour, A.
700 1 2 _aChurch, G.M.
856 4 0 _uhttps://drive.google.com/file/d/14KG349UEHYc5WFS7l2_wCD6l6WwoAMZv/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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